Have you tried checking if there is a short between 12V and ground, without the supply?
And check if you have continuity between jack and board. Just to check that the wires are correctly soldered. Happens with everybody.
If that does not work, it is time to check the circuit by areas. You can unsolder the trace that goes to the 555, to cut the 12V connection and check the if you have 12V at the tubes. For this test, you can even measure it with the tubes disconnected. We just want to know if there is a problem with the tubes or with the 555. If the first section of the tubes is OK, you can add the tubes and see if they light up. If it still works, the problem is with the SMPS. If not, you have bad tubes.
To check the tubes, measure the resistance of the heaters. It should be very low, but higher than 1 to 4R. If you have a low resistance at the filaments, the tube is shorted and you will have to fin another one. Normally they do not fail that way, but who knows.
Which kind of supply are you using? Does it have any kind of protection? I had some good supplies which would shut off when shorted. In some cases the heaters of the tubes look like a short. In your case, I think it will be OK because they are in series.
If it works with the tubes, but not with the SMPS, try disconnecting stuff from the 555, if it's possible. Ideally you would test if the 555 is working, without the mosfet and diode. Just to see if there is something wrong with this part of the circuit. If the 555 is socketed, you can remove it and measure if you get 12V without it (bad 555) or if it is still at 0V.
You can check if the Mosfet (IRF740) is good by measuring the resistance between pins. It should be around 740R, if I am not mistaken. A shorted mosfet is the most common problem with the SMPS I build, you just need to short the HV line and gnd and it kills the mosfet and the 555.
Good luck! This is a really nice amplifier.
PS.: I checked your board, and there are some suspicious points...the schematic was mirrored to be easier to compare. Check at the points with red circles, if there is a short between traces.
I would suggest that you use less solder, or if you can, remove some of it. The large blobs do not help, most of the time, when it looks like a nice sphere, you have actually a bad joint.
